Antimicrobial resistance (AMR) is a critical global health issue addressed through metagenomics, which examines resistomes in diverse microbial environments. Using Oxford Nanopore’s long reads, antibiotic resistance genes are identified in raw data through shotgun metagenomic and whole genome sequencing, leveraging the Comprehensive Antibiotic Resistance Database (CARD).

Metagenomics enables the assessment of microbial diversity and quantities within samples. While 16S rRNA gene identification at ~1500 bp is common, many modern sequencing methods use only a ~450 bp fragment, leading to inaccuracies. Oxford Nanopore’s long reading technology enhances species identification by reading the full 1500 bp gene, advancing the field. Metagenomic analyses offer extensive options for detailed exploration of diverse sample types or components.

Whole genome analysis involves a thorough study of an organism’s entire genome and its functional genes. This method detects variants – differences from the reference genome – along with antimicrobial resistance genes, secondary metabolite genes, and virulence genes in the annotated genome. It also includes tools like alignment images, circular genome depictions, genome comparison with close subspecies, polymorphism tables.

Experience our Amplicon Sequencing Service—a tailored genetic analysis solution crafted to reveal precise insights from DNA or RNA samples. Through targeted amplification and sequencing of specific regions, we deliver focused, high-resolution data ideal for identifying mutations, uncovering genetic diversity, and tracing variations. Our state-of-the-art technology ensures precise outcomes, even with limited genetic material, enabling swift and cost-effective decision-making. Whether in medical advances or environmental exploration, our Amplicon Sequencing Service paves the way for efficient and impactful genetic analysis.