Oxford Nanopore Technologies

Analysis of anything by anyone, anywhere.

World’s Most Accesible High-Yield Sequencing Technology

Oxford Nanopore Technologies

Through the vision of “the analysis of anything, by anyone, anywhere” Oxford Nanopore Technologies (ONT) put forth a new generation of sensing technology. Having utilized this technology in numerous fields and experienced its implications firsthand, we’re marveled at the impact. Thus, taking on the responsibility to distribute all the cutting-edge ONT devices, and products merged with our vision.

Nanopore sequencing is the only technology that offers scientific researchers…

Devices

MiniION MK1B/MK1C

GridION

PromethION

Voltrax

MinION MK1C/MK1B

GridION

PromethION

VolTRAX

The only portable, real-time devices for DNA and RNA sequencing, putting you in control of your sequence data. Sequence immediately, not wait. Sequence outside a lab. 10–20Gb per 48 hrs. Avoid CapEx investments.

WHY CHOOSE MINION?

  • Pocket-sized, portable device for biological analysis

  • Up to 512 nanopore channels

  • Simple 10-min sample prep available

  • Real-time analysis for rapid, efficient workflows

  • Adaptable to direct DNA or RNA sequencing

  • MinIT available to support IT/software needs

Compact benchtop device designed to run and analyse up to five Flongle or MinION Flow Cells. Scale up from MinION. Offer nanopore sequencing as a service.

WHY CHOOSE GRIDION?

  • Run up to five independently addressable Flongle or MinION Flow Cells

  • As much as 150 Gb of data — streamed in real time for immediate analysis

  • Powerful onboard data processing and analysis, minimising IT requirements

  • Small footprint, suitable for any lab

Large-scale, long-read, direct DNA and RNA sequencing. Offer nanopore sequencing as a service. Very large data volumes projects (Tb). On-demand sequencing for large numbers of samples.

WHY CHOOSE PromethION?

  • Run up to 48 independently addressable, high-capacity PromethION Flow Cells

  • Generate terabases of data — streamed in real time for immediate analysis

  • Alleviate data analysis bottlenecks

Automatic library preparation: VolTRAX is designed as an alternative to a range of lab equipment, enabling the preparation of your biological samples, ready for analysis, hands-free.

WHY CHOOSE VOLTRAX?

  • Only minutes of hands-on time, even for novel/complex experiments

  • Take sample prep out of the lab

  • Develop own sample and library prep methods, including fluorescence detection and incubations

  • Select your protocol, load, and leave

03Unrestricted read length

03Real time

03Portable

03Powerful

03Flexible

Run up to five independently addressable Flongle or MinION Flow Cells.

03High Throughput

As much as 250 Gb* of data — streamed in real time for immediate analysis.

03Intergrated Compute

Powerful onboard data processing and analysis, minimising IT requirements.

03Compact

Small footprint, suitable for any lab.

03On Demand Sequencing

Run up to 48 independently addressable, high-capacity PromethION Flow Cells.

03Real time

Alleviate data analysis bottlenecks.

03Portable

Generate terabases of data — streamed in real time for immediate analysis.

03Automated

Only minutes of hands-on time, even for novel/complex experiments.

03Programmable

Develop own sample and library prep methods, including fluorescence detection and incubations.

03Portable

Take sample prep out of the lab.

03Simple

Select your protocol, load, and leave.

Flow Cells

PromethION Flow Cells

MinION&GridION Flow Cells

Flongle Flow Cell

PromethION Flowcell

MinION & GridION Flowcell

Flongle (Flow Cell)

 Up to 290 GB* of Data in 72 hours

 

The PromethION Flow Cell contains up to 2675 nanopore channels for sequencing DNA or RNA in real time.

 

Purchase options:

  • Available as packs, contains 4 PromethION flow cells
  • Buy as annual or top-up packs to meet your requirements
  • Available as R9.4.1, R10.4 and our latest chemistry, R10.4.1

 

 

Up to 50 GB* of Data in 72 hours

 

The MinION and GridION Flow Cell contains up to 512 nanopore channels for sequencing DNA or RNA in real time.

 

Purchase options:

  • Buy single flow cells or save money by buying in bulk
  • Available as R9.4.1, R10.4 and our latest chemistry, R10.4.1

 

 

Up to 2.8 GB* of Data in 16 hours

 

The Flongle Flow Cell contains the proprietary sensor array required for all 1D experiments.

 

Purchase Options:

  • Flongle adapters are available through the Flongle StarterPack
  • Available as R9.4.1 and our latest chemistry, R10.4.1

 

Sequencing Kits

Ultra-Long DNA Sequencing Kit V14 

An overnight method to prepare ultra-high molecular weight DNA for sequencing ultra-long reads (N50 >50 kb).

This uses our latest Kit 14 chemistry.

  • Preparation time: ~200 minutes + overnight elution
  • Read length: N50 >50 kb
  • Input amount: 6 million cells

Rapid Barcoding Kit 96 V14 

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples.

This uses our latest Kit 14 chemistry.

  • Preparation time: 60 minutes
  • Read length: Random distribution, dependent on input fragment length
  • Input amount: 50 ng dsDNA per sample

Rapid PCR Barcoding Kit 24

Rapid and simple method of preparing up to 24 barcoded samples for sequencing from 1-5 ng of gDNA using PCR.

This uses our latest Kit 14 chemistry.

Product lead time: 6-8 weeks

  • Preparation time: 15 minutes + PCR
  • Read length: ~2 kb
  • Input amount: 1-5 ng gDNA per sample

Rapid Sequencing Kit V14 

A simple and rapid library preparation method for genomic DNA optimised for speed with improved modal raw read accuracy.

This uses our latest Kit 14 chemistry.

  • Preparation time: 10 minutes
  • Read length: Random distribution, dependent on input fragment length
  • Input amount: 100 ng dsDNA

Native Barcoding Kit 96 V14

A versatile method of preparing barcoded sequencing libraries optimised for median raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This uses our latest Kit 14 chemistry.

Product lead time: 6-8 weeks

  • Preparation time: 140 minutes
  • Read length: = to fragment length
  • Input amount: 400 ng per sample of gDNA, 200 fmol per sample (130 ng for 1 kb amplicons)

Rapid Barcoding Kit 24 V14 

Simple and rapid library preparation, with barcoding for up to 24 gDNA samples.

This uses our latest Kit 14 chemistry.

  • Preparation time: 60 minutes
  • Read length: Random distribution, dependent on input fragment length
  • Input amount: 50 ng dsDNA per sample

Native Barcoding Kit 24 V14

A versatile method of preparing barcoded sequencing libraries optimised for median raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

Product lead time: 6-8 weeks

This uses our latest Kit 14 chemistry.

  • Preparation time: 140 minutes
  • Read length: = fragment length
  • Input amount: 400-1000 ng for gDNA. 200 fmol (130 ng for 1 kb amplicons) for amplicon input

Ligation Sequencing Kit XL V14 

A versatile sequencing kit optimised for median raw read accuracy of Q20+ (99%+) with long read singleplex samples. Multiple samples can be processed simultaneously.

This uses our latest Kit 14 chemistry.

  • Preparation time: 120 minutes
  • Read length: = fragment length
  • Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR, 100-200 fmol of  amplicons or cDNA

Ligation Sequencing Kit V14 

SQK-LSK114

A versatile sequencing kit optimised for median raw read accuracy of Q20+ (99%+) and long read singleplex samples.

This uses our latest Kit 14 chemistry.

  • Preparation time: 60 minutes
  • Read length: = fragment length
  • Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR,100-200 fmol of  amplicons or cDNA

PCR-cDNA Barcoding Kit

SQK-PCB111.24

Identification and quantification of full-length transcripts with highest output, with barcodes for up to 24 samples.

This uses our Kit 11 chemistry. 

  • Preparation time: 210 minutes + PCR
  • Read length: Enriched for full-length cDNA during PCR
  • Input amount: 4 ng poly(A)+ RNA, or 200 ng total RNA per sample

Multiplex Ligation Sequencing Kit

SQK-MLK111.96-XL

A sequencing kit optimised for output and PCR-free sequencing of 2-3 samples for Whole Genome Sequencing (WGS).

This uses our Kit 11 chemistry.

  • Preparation time: 135 minutes
  • Read length: = Fragment length
  • Input amount: 1000 ng dsDNA per sample

cDNA-PCR Sequencing Kit 

SQK-PCS111

Identification and quantification of full-length transcripts with highest output.

This uses our Kit 11 chemistry.

  • Preparation time: 210 minutes + PCR
  • Read length: Enriched for full-length cDNA during PCR
  • Input amount: 4 ng poly(A)+ RNA, or 200 ng total RNA

cDNA-PCR Sequencing Kit 

C19MAXI

A simple, scalable and rapid method for sequencing up to 9,216 SARS-CoV-2 samples.

COVID Midi: 2304 samples

C19MIDI

A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples.

COVID Mini: 576 samples

C19MINI

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.

Direct RNA Sequencing Kit

SQK-RNA002

Prepare RNA for direct RNA nanopore sequencing.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit.

  • Preparation time: 115 minutes
  • Read length: = RNA length
  • Input amount: 500 ng total RNA, 50 ng poly(A)+ RNA

Rapid Barcoding Kit 96

SQK-RBK110.96

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples. We recommend using our most recent version: Rapid Barcoding Kit 96 V14 (SQK-RBK114.96).

This uses Kit 10 chemistry.

  • Preparation time: 60 minutes
  • Read length: Random distribution, dependent on input fragment length
  • Input amount: 50 ng dsDNA per sample

16S Barcoding Kit 1-24

SQK-16S024

Genus-level bacterial identification with barcoding for up to 24 samples.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit.

  • Preparation time: 10 minutes + PCR
  • Read length: = full-length 16S gene (~1.5kb)
  • Input amount: 10 ng dsDNA per sample

Cas9 Sequencing Kit

SQK-CS9109

Targeted sequencing of genomic regions using CRISPR-Cas9.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit.

  • Preparation time: 110 minutes
  • Read length: = fragment length
  • Input amount: 1–10 µg of dsDNA

Expansion Kits

Flow Cell Priming Kit XL

EXP-FLP004-XL

Additional flow cell priming reagents for our V14 kits.

Product lead time: 6-8 weeks

Note: This is only compatible with V14 chemistry.

    Native Barcoding Auxiliary Kit V14

    EXP-NBA114

    Additional library preparation reagents for the Native Barcoding Kit 24 and 96 V14 (SQK-NBD114.24 and SQK-NBD114.96).

    Note: This is only compatible with V14 chemistry.

    Rapid Adapter Auxiliary V14

    EXP-RAA114

    Additional Rapid Adapter and Adapter Buffer to enable customers to maximise the use of Rapid Barcoding Kits 24 and 96 V14 (SQK-RBK114.24 and SQK-RBK114.96).

    Note: This is only compatible with V14 chemistry.

      Flow Cell Priming Kit

      EXP-FLP004

      Additional flow cell priming reagents for our V14 kits.

      Note: This is only compatible with V14 chemistry.

        Sequencing Auxiliary Vials V14 

        EXP-AUX003

        The Sequencing Auxiliary Vials contain Sequencing Buffer (SB), Elution Buffer (EB), Library Solution (LIS) and Library Beads (LIB) with V14 flow cell priming reagents: Flow Cell Flush (FCF) and Flow Cell Tether (FCT).

        Note: This is only compatible with V14 chemistry.

          RNA Control Expansion

          EXP-RCS001

          RNA Control Sample to support control runs for cDNA kits.

            RAP T Expansion 

            EXP-RAP111

            Additional Rapid Adapter T (RAP T) to maximise the use of the PCR-cDNA Barcoding Kit (SQK-PCB111.24).

            Product lead time: 6-8 weeks

            Note: This is only compatible with Kit 11 chemistry.

            Short Fragment Eliminator Expansion

             EXP-SFE001

            An expansion pack designed for size selection of high molecular weight DNA samples to progressively deplete short fragments of <25 kb.

            Flow Cell Wash Kit XL

            EXP-WSH004-XL

            The Flow Cell Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells on a higher scale.

            This is compatible with all sequencing kits.

            Midnight RT PCR Expansion

            EXP-MRT001

            An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.

            Product lead time: 6-8 weeks

            This is compatible with Kit 10 and Kit 14 chemistry.

            • Preparation time: 80 minutes + PCR

            Flow Cell Wash Kit

            EXP-WSH004

            The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

            This is compatible with all sequencing kits.

            Control Expansion Kit

            EXP-CTL001

            Lambda DNA (LMD). Pack of six vials.

            This is compatible with all DNA sequencing kits.

            PCR Expansion

            EXP-PCA001

            PCR Primers (PRM) and PCR Adapters (PCA).

            PCR Barcoding Expansion 1-96

            The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries.

            • Preparation time: 150 minutes
            • Read length: = to fragment length post PCR
            • Input amount: 1000 ng dsDNA

            PCR Barcoding Expansion 1-12

            EXP-PBC001

            The PCR Barcoding Expansion 1-12 (EXP-PBC001) allows the pooling and running together of multiple sequencing libraries.

            • Preparation time: 150 minutes
            • Read length: = to fragment length post PCR
            • Input amount: 1000 ng dsDNA

            LFB Expansion

            EXP-LFB001

            Additional Long Fragment Buffer (LFB).

            Product lead time: 6-8 weeks

            Note: This is compatible with all kit chemistries.

             

            SFB Expansion

            EXP-SFB001

            Additional Short Fragment Buffer (SFB).

            Note: This is compatible with all kit chemistries.

            Contact us now!

            From customized bioinformatics analysis to innovative software tools, and Next Genereation Sequencing devices we provide everything you need to accelerate your research. Explore our services and products today. Contact us to schedule a meeting with our experts and achieve your research goals. Together, we can make a difference. 

            Expert Consultancy

            When you contact us, you can discover the most suitable solutions for your projects through one-to-one meetings with our expert consultants.

            Customized Services

            With services and products tailored specifically to your needs, we will accelerate your research processes and help you achieve your goals in less time.