Ultra-Long DNA Sequencing Kit V14 

An overnight method to prepare ultra-high molecular weight DNA for sequencing ultra-long reads (N50 >50 kb).

This uses our latest Kit 14 chemistry.

• Preparation time: ~200 minutes + overnight elution
• Read length: N50 >50 kb
• Input amount: 6 million cells

Rapid Barcoding Kit 96 V14 

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples.

This uses our latest Kit 14 chemistry.

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample

Rapid Sequencing Kit V14 

A simple and rapid library preparation method for genomic DNA optimised for speed with improved modal raw read accuracy.

This uses our latest Kit 14 chemistry.

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 100 ng dsDNA

Native Barcoding Kit 96 V14

A versatile method of preparing barcoded sequencing libraries optimised for median raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This uses our latest Kit 14 chemistry.

Product lead time: 6-8 weeks

• Preparation time: 140 minutes
• Read length: = to fragment length
• Input amount: 400 ng per sample of gDNA, 200 fmol per sample (130 ng for 1 kb amplicons)

Native Barcoding Kit 24 V14

A versatile method of preparing barcoded sequencing libraries optimised for median raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

Product lead time: 6-8 weeks

This uses our latest Kit 14 chemistry.

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 400-1000 ng for gDNA. 200 fmol (130 ng for 1 kb amplicons) for amplicon input

Ligation Sequencing Kit XL V14 

A versatile sequencing kit optimised for median raw read accuracy of Q20+ (99%+) with long read singleplex samples. Multiple samples can be processed simultaneously.

This uses our latest Kit 14 chemistry.

• Preparation time: 120 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR, 100-200 fmol of  amplicons or cDNA

PCR-cDNA Barcoding Kit

SQK-PCB111.24

Identification and quantification of full-length transcripts with highest output, with barcodes for up to 24 samples.

This uses our Kit 11 chemistry. 

• Preparation time: 210 minutes + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly(A)+ RNA, or 200 ng total RNA per sample

Multiplex Ligation Sequencing Kit

SQK-MLK111.96-XL

A sequencing kit optimised for output and PCR-free sequencing of 2-3 samples for Whole Genome Sequencing (WGS).

This uses our Kit 11 chemistry.

• Preparation time: 135 minutes
• Read length: = Fragment length
• Input amount: 1000 ng dsDNA per sample

cDNA-PCR Sequencing Kit 

C19MAXI

A simple, scalable and rapid method for sequencing up to 9,216 SARS-CoV-2 samples.

COVID Midi: 2304 samples

C19MIDI

A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples.

COVID Mini: 576 samples

C19MINI

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.

Direct RNA Sequencing Kit

SQK-RNA002

Prepare RNA for direct RNA nanopore sequencing.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit.

• Preparation time: 115 minutes
• Read length: = RNA length
• Input amount: 500 ng total RNA, 50 ng poly(A)+ RNA

16S Barcoding Kit 1-24

SQK-16S024

Genus-level bacterial identification with barcoding for up to 24 samples.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit.

• Preparation time: 10 minutes + PCR
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng dsDNA per sample

Cas9 Sequencing Kit

SQK-CS9109

Targeted sequencing of genomic regions using CRISPR-Cas9.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit.

• Preparation time: 110 minutes
• Read length: = fragment length
• Input amount: 1–10 µg of dsDNA

Flow Cell Priming Kit XL

EXP-FLP004-XL

Additional flow cell priming reagents for our V14 kits.

Product lead time: 6-8 weeks

Note: This is only compatible with V14 chemistry.

Native Barcoding Auxiliary Kit V14

EXP-NBA114

Additional library preparation reagents for the Native Barcoding Kit 24 and 96 V14 (SQK-NBD114.24 and SQK-NBD114.96).

Note: This is only compatible with V14 chemistry.

Flow Cell Priming Kit

EXP-FLP004

Additional flow cell priming reagents for our V14 kits.

Note: This is only compatible with V14 chemistry.

Sequencing Auxiliary Vials V14 

EXP-AUX003

The Sequencing Auxiliary Vials contain Sequencing Buffer (SB), Elution Buffer (EB), Library Solution (LIS) and Library Beads (LIB) with V14 flow cell priming reagents: Flow Cell Flush (FCF) and Flow Cell Tether (FCT).

Note: This is only compatible with V14 chemistry.

RAP T Expansion 

EXP-RAP111

Additional Rapid Adapter T (RAP T) to maximise the use of the PCR-cDNA Barcoding Kit (SQK-PCB111.24).

Product lead time: 6-8 weeks

Note: This is only compatible with Kit 11 chemistry.

Short Fragment Eliminator Expansion

 EXP-SFE001

An expansion pack designed for size selection of high molecular weight DNA samples to progressively deplete short fragments of <25 kb.

Midnight RT PCR Expansion

EXP-MRT001

An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.

Product lead time: 6-8 weeks

This is compatible with Kit 10 and Kit 14 chemistry.

• Preparation time: 80 minutes + PCR

Flow Cell Wash Kit

EXP-WSH004

The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

This is compatible with all sequencing kits.

PCR Expansion

EXP-PCA001

PCR Primers (PRM) and PCR Adapters (PCA).

PCR Barcoding Expansion 1-96

The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries.

• Preparation time: 150 minutes
• Read length: = to fragment length post PCR
• Input amount: 1000 ng dsDNA

LFB Expansion

EXP-LFB001

Additional Long Fragment Buffer (LFB).

Product lead time: 6-8 weeks

Note: This is compatible with all kit chemistries.

SFB Expansion

EXP-SFB001

Additional Short Fragment Buffer (SFB).

Note: This is compatible with all kit chemistries.