Bacterial Whole Genome Analysis

Whole Genome Sequencing (WGS)

Whole genome analysis is a method of genetic analysis that provides a comprehensive examination of an organism’s entire genome and functional genes. With this analysis, changes in positions where the genome of the organism differs from the reference genome, namely variants, are detected. Antimicrobial resistance genes, secondary metabolite genes and virulence genes are detected in the annotated genome.

Below are key components and analyses involved in our genome research, providing a comprehensive view of genomic data and its implications:

Alignment image
Image of circular genome with functional genes
Genome comparison analysis with close subspecies (BRIG)
Polymorphism table
Antimicrobial resistance genes
Secondary metabolite genes
Virulence genes
LASTZ-Mauve multi-sample genome alignment analysis

Massive Bioinformatics is at your disposal with whole genome sequencing analysis services for microbial organisms. In whole genome sequencing analyzes, we provide services for comprehensive, more accurate and inclusive studies by utilizing both long reading and short reading technologies. Whole genome of organisms such as bacteria, archaea, fungi and parasites, comperative genomics, examination of polymorphic structures, anotations and many more analyzes according to your needs.

Massive Bioinformatics provides solutions for your project in whole genome sequencing analyzes. We carry out analyzes with short or long readings and hybrid readings for your organisms whose reference is known or de novo sequencing. Antimicrobial resistance genes, gene anotations, polymorphism statistics, analyzes for metabolic gene clusters, RNA varieties, plasmid genome (if any), comparative analysis of different strains and many other analyzes can be consulted to us for your needs.

Bacteria, Archaea, Fungi and Viral Genome Analysis

More Accurate Results with Long Reading

Variations between Strains by Comparative Analysis

Project Based Analysis and Methodology

Frequently Asked Questions (FAQ)

How should I send my samples?

You can send your samples as pure culture or total genomic DNA. If you have Gram staining information, you are encouraged to share it. The amount of sample and DNA to be sent may vary depending on the study. Therefore, please read the Sample Acceptance Criteria file carefully and contact your Sales Responsible.

Is PCR applied in genomic DNA sequencing?

No, in this sequence the native DNA is sequenced directly. In this way, you may also be able to identify methylations on genomic DNA. Please contact your Sales Contact for further information.

Can you study every bacterium?

Yes, but pathogenic bacteria are not accepted in our laboratory. Please make sure that the samples you submit as pure cultures are not human pathogens. If sequencing for pathogenic bacteria will be performed in your study, the sample should be submitted as genomic DNA only after DNA isolation. Pathogenic bacteria will not be included in the study.

Antimicrobial Resistance Analysis (AMR)

Antimicrobial resistance (AMR) is an important global health problem. Metagenomics enables the analysis of resistomes in various microbial ecosystems.

With shotgun metagenomic sequencing and whole genome sequencing, antibiotic resistance genes are detected with long reads using Oxford Nanopore Technologies. Resistome detection is performed within raw data using the Comprehensive Antibiotic Resistance Database (CARD).

Frequently Asked Questions (FAQ)

What is the difference between bacterial whole genome sequencing and bacterial whole genome AMR analysis?

The laboratory steps of the two methods are exactly the same. AMR analysis is a specialized analysis performed alongside a bacterial whole genome study. The same data set can be used for both analyses.