Metagenomic Analyzes
Massive Bioinformatics specializes in metagenomic analyses that make use of Oxford Nanopore technology. We provide the following services based on your project requirements:
Comparative Analysis and Data Visualization:
We provide comparative analysis of samples for population research and biomarker discovery. We also give visually appealing graphical presentations that are relevant to the subject of your research.
Metagenomic analyzes of bacteria and archae groups with 16s rRNA gene, fungi with ITS region, and eukaryotic organisms with 18S rRNA gene are performed by using barcode genes for different living groups. It is possible to use different databases according to the content of the study, combinations with different workflows to increase the accuracy of the results are among the services we provide.
Custom Database and Workflow:
To ensure the maximum possible accuracy of the results, we can adapt the databases and workflows employed based on the specific requirements of your study. Metagenomic analyzes of bacteria and archae groups with 16s rRNA gene, fungi with ITS region, and eukaryotic organisms with 18S rRNA gene are performed by using barcode genes for different living groups.
Comprehensive Metagenomic Analysis:
We perform metagenomic analyses on bacteria and archaea (using the 16S rRNA gene), fungus (using the ITS region), and eukaryotic species (using the 18S rRNA gene). This is accomplished by employing barcode genes unique to each group.
Species Identification:
Using Oxford Nanopore technology, we deliver high-resolution species identification by sequencing the full 16S rRNA gene (1500 bp) not only the hypervariable V3-V4 region (450 bp), allowing for greater taxonomic precision. This strategy dramatically reduces the error rate associated with short-read sequencing technologies that only target ~450 bp of the gene.
Species Diversity and Quantity Analysis
Comparative Taxonomic Analysis
PCA Analysis of Samples
Principal Coordinate and Component Analyzes
Alpha – Beta Diversity Analyzes
Shotgun Metagenomics
Shotgun Metagenomic analysis makes possible to access all nucleic acid information contained in a sample. All of the total genomic material obtained by this method is sequenced and then the desired information is reached with the databases for target organism groups or genes. The amount of data to be obtained and the methods of analysis vary according to the content of the sample and the desired information. Massive Bioinformatics provides shotgun metagenome analysis with both short readings and long readings.
All Genes Found in the Environment
Viral Metagenomics
More Accurate Results with Long Reading
Whole Genome Construction from Metagenomik Data
Frequently Asked Questions (FAQ)
Can I send a sample directly to your laboratory for this service?
Yes, you can. However, be sure to contact your Sales Contact to share detailed information about the sample quantity and what you want to find specifically (viruses, bacteria, etc.).
Does the presence of host DNA in my sample affect my analysis?
Yes, in the Shotgun metagenomics approach, the total DNA in the sample is isolated and sequenced directly. If host DNA is present in your sample, depending on the sample type, the majority of the data you receive will align to the host genome. Please contact your Sales Contact for our host DNA removal related solutions.
Species Diversity and Identification
Long Reads
Whole Genome Analysis
Functional Analyzes
SSu – LSu Metagenomics Analyses
Metagenomics analyzes make possible to determine the diversity and amounts of microorganisms in a sample. Identification of bacteria with the 16S rRNA gene with a length of ~ 1500 bp is now a standardized process. However, most of the new generation sequencing methods used only a portion of this gene which is approximately ~ 450 bp in length and therefore produce erroneous results. Oxford Nanopore enhanced the resolution of species identification by reading the entire 1500 bp 16S rRNA gene thanks to its long reading technology and opened a new page in this field. Metagenomic analyzes provide possibilities for detailed examination of a wide variety of sample types, or components of a sample.
More Accurate Metagenome Results with Long Reading
Bacteria, Archaea, Eukaryotes and Fungi
Metagenomics with 16S rRNA, ITS, 18S rRNA Genes
Comperative Metagenomics Analyzes
Frequently Asked Questions (FAQ)
Can I also send my samples as amplicons?
Yes, on the long-read based Oxford Nanopore Platform, we are analyzing 16S or 23S regions for bacterial distributions, ITS and 18S regions for fungi and eukaryotes. You can send your samples as amplicons by sharing the sequences, amplicon lengths and gel confirmation images of the primers you use.