Amplicon Sequencing

Amplicon Sequencing
Discover with our Amplicon Sequencing Service – a targeted genetic analysis solution designed to unlock precise insights from DNA or RNA samples. By selectively amplifying and sequencing specific regions of interest, we provide you with focused, high-resolution data, perfect for pinpointing mutations, unraveling genetic diversity, and tracking variations. Our cutting-edge technology ensures accurate results even from limited genetic material, empowering you to make informed decisions swiftly and cost-effectively. From medical breakthroughs to environmental exploration, our Amplicon Sequencing Service is your pathway to streamlined, impactful genetic analysis.
Whole Amplicon Sequence with Long Read Technology
Primer Design For Desired Regions
Multiple Region Analysis in a Single Run
Project Based Analysis and Methodology
Frequently Asked Questions (FAQ)
How should I send my sample?
In order to sequence the specific amplicons you are working with, you must submit your PCR products to us as specified in the Sample Acceptance Conditions file. When filling out the Sequencing Service Information form, please do not forget to include the primer used and amplicon length.
Which amplicon lengths are suitable for the amplicon sequencing service?
There is no upper limit for amplicon length when sequencing with Oxford Nanopore Technology. What you need to pay attention to is the lower limit. Sequencing is performed using amplicons with a minimum length of 200 base pairs. It is not recommended to work with amplicons shorter than 200 base pairs.
Can I study any region I want?
We can work with all amplicons you send us, but the products to be sequenced must be submitted as PCR products. PCR amplification is not performed by us except for certain regions (16S V1-V9, ITS, 23S…). The amplified products are taken directly to sequencing.
What should I consider when sending a sample for amplicon sequencing service?
Make sure you provide all the following information:
– Primer sequences used – preferably in excel format should be uploaded to the Sequencing Service Information Sheet.
– Amplicon concentrations and volumes – preferably in excel format to be uploaded to the Sequencing Service Information Sheet.
– Gel image showing amplicon length and amplification success – Visual Sequencing Service Information Form.
All information must be uploaded to the form for all samples. Sample names must be clearly listed.