Scientific rigor driving innovation since 2003
About Molzym
Molzym is one of the leading providers of culture-independent molecular solutions, offering a wide range of CE IVD-marked molecular diagnostic kits, host DNA depletion technology MolYsis™, and ultra-pure PCR reagents and tests. Molzym’s technology enables the direct detection of microbes from body fluids, tissues, and swabs using PCR or NGS-based methods. Pathogens can be identified within hours, significantly improving patient management through rapid, pathogen-targeted antimicrobial treatment.
Diagnostics
SepsiTest™-UMD CE IVD
Detection of robust, unculturable, and antibiotic-inhibited pathogens
SepsiTest™-UMD is a CE IVD-marked assay kit designed for the culture-independent detection and identification of bacterial and fungal pathogens in body fluids, tissues, and swab samples for routine diagnostics.
Characters and benefits:
- A single protocol for a wide variety of clinical samples (fluids, tissues, swabs)
- Detection of robust, unculturable, and antibiotic-inhibited pathogens
- Identification of over 1,350 microorganisms to date
- Extraction of enriched microbial DNA
- PCR or Real-Time PCR tests targeting the 16S and 18S rRNA genes of bacteria and fungi
- Identification of bacterial and fungal species through Sanger sequencing
- Includes PCR and extraction controls
- All reagents are guaranteed to be DNA-free
Sample analysis up to 10 ml with Add-On 10
Automated Pathogen Diagnosis
Fully Automated Pathogen Diagnosis
Automatic pathogen enrichment, DNA isolation, and broad-range 16S & 18S PCR tests on the SelectNA™plus robot.
Semi-Automated Pathogen Diagnosis
A combination of manual pathogen enrichment with automated DNA isolation and broad-range 16S & 18S PCR tests.
Micro-Dx™ CE IVD
Characters and benefits:
- Completely automated human DNA depletion and microbial DNA extraction
- Robotic isolation of bacterial and fungal DNA
- Low hands-on time (10-15 min)
- Just one protocol for a diversity of clinical specimens
- Culture-independent diagnosis of growing and non-growing pathogens
- Sequencing identification of pathogens from 160 bacterial genera
- Exact discrimination of Candida, Cryptococcus and other yeast species, Aspergillus
- Flexibility: processing of 1-12 samples per run
- Controlled DNA-free reagents and consumables
- Extraction and PCR run controls included
Deep-Freeze Sample Storage
Long-term storage of your valuable samples – UMD-Tubes are the perfect solution for long-term stabilization of intact microorganisms in clinical and other samples. UMD-Tubes can be ideally combined with our CE IVD kits SepsiTest™-UMD and Micro-Dx™.
Characters and benefits:
- Stabilization of live bacteria and fungi in fluid samples
- Protection of pathogens from freeze-thaw damage
- Vial and cryo-protectant are free of contaminating microbial DNA
- Flexible sample volume: 0.4 to 2 ml
UMD-Tubes are vials containing a cryo-protectant for long-term storage of clinical and other fluid samples at -70 to -80 °C. The sample storage conditions are optimal for the analysis of samples at a later time, e.g. for studies and evaluations. Often, sampling and storage are critical steps, especially if assays are targeting intact cells. UMD-Tubes prevent the rupture of microorganisms by freeze-thaw events and guarantee full performance of the diagnosis without loss of sensitivity, even after months of storage.
Life Science
Pathogen DNA Enrichment and Isolation
MolYsis™ kits are ideal for the supply of enriched microbial DNA for the direct analysis of pathogens in human and animal samples. The kits are evaluated for all sorts of body fluids like blood, CSF, synovial aspirates, BAL and other specimens.
Characters and benefits:
- Depletion of host (human/animal) DNA from body fluids
- Enrichment of bacterial and fungal DNA
- Removal of PCR inhibitors
- Broad-range lysis of Gram-positive, Gram-negative bacteria and fungi
- Buffers and reagents are contamination-free
- 0.2 to 1 ml or 5 ml sample volumes
MolYsis™ is a unique system of targeted isolation of microbial DNA from complex clinical samples such as blood, aspirates and other body fluids. The selective lysis of host cells and the degradation of the released host DNA avoid unspecific primer binding and thereby greatly enhance the sensitivity and specificity of PCR or Real-Time PCR analysis of pathogens. For detailed information see Technology. The kit series allows sample processing in the range of 0.2 to 1 ml or 5 ml.
Automated Microbial DNA Enrichment and Extraction
The SelectNA™plus robotic system is an automated solution for the enrichment and isolation of microbial DNA. Its enclosed environment is ideal for minimizing the risk of airborne contamination and user errors.
The robot is operated with the MolYsis-SelectNA™plus kit and validated for 1 ml fluid samples, swabs and tissue biopsies. The process is based on an innovative vacuum-driven protocol for host DNA depletion and pathogen enrichment followed by microbial DNA isolation providing an optimal solution for e.g. low load samples with only small amounts of microbial DNA. The isolated DNA can be used with any molecular assay for the analysis of bacterial or fungal genes, genomes or metagenomes.
Characters and benefits:
- Innovative walk-away pathogen DNA extraction
- Host-DNA-depletion technology
- DNA-free consumables and reagents
- Vacuum-driven system
- UV decontamination
- Validated for primary sterile body fluids, swabs and tissues
- Bench-top instrument
- Flexible: 1 to 12 samples at a time
DNA-Free PCR Reagents & Assays
Try Molzym’s contamination-free PCR/Real-Time PCR products for the sensitive detection of bacterial and fungal DNA. Available are reagents for custom assay development and complete 16S and 18S assays.
PCR and Real-Time PCR Reagents
Polymerases, water and master mixes are manufactured free of microbial DNA and are high in amplification activity over 40 cycles. Master mixes contain all reagents for assays to be used with custom primers.
Complete assays are available for the sensitive PCR/Real-Time PCR detection of parts of 16S and 18S rRNA genes of eubacteria and fungi, respectively. Amplicon sequencing primers enable their identification.
PCR and Real-Time PCR Reagents
Our special purification technology removes traces of DNA from PCR reagents. Taq and master mixes unfold highest sensitivity and reliability of detection of microbial DNA in assays employing your own primers.
A series of kits is especially dedicated to the sensitive and specific detection of bacterial and fungal DNA in samples. All reagents are manufactured DNA-free with respect to microbial DNA contaminations. Likewise, the reagents are highly active in their amplification performance, allowing up to 40 amplification cycles without background in negative controls. DNA-free MolTaq 16S/18S and Hot MolTaq 16S/18S are the Taq DNA polymerases of choice if you experience contamination problems with other standard Taqs. The new developed Hot MolTaq 16S/18S combines the favourable characters of MolTaq 16S/18S with hot start amplification and thereby increased specificity.
Master mixes contain pre-assembled reagents necessary for PCR, including dNTPs, buffer, and Mg2+ (3mM final concentration). Mastermix 16S/18S Basic is a ready-to-go master mix – just add your validated PCR primers and probes. Mastermix 16S/18S Dye is the master mix of choice if you want to run Real-Time PCRs with your primers using an intercalating dye.
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From customized bioinformatics analysis and innovative software tools to Next Genereation Sequencing devices we provide everything you need to improve your research. Explore our services and products today. Contact us to schedule a meeting with our experts about your research projects. Together, we can make a difference.
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Customized Services
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